Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae and Pichia pastoris X-33 cells | Candolleomyces aberdarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
0.143 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
0.201 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
0.354 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
0.357 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
0.397 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
0.411 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
0.434 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
1.277 | - |
veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
2.259 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
4.006 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
6.675 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Candolleomyces aberdarensis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candolleomyces aberdarensis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 9% glycosylation degree | Candolleomyces aberdarensis |
glycoprotein | mutant S61A/L79I/A252L of isoform UPO-I has 14% glycosylation degree | Candolleomyces aberdarensis |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 75-pg gel filtration | Candolleomyces aberdarensis |
ammonium sulfate precipitation, phenyl Sepharose column chromatography, Mono Q column chromatography, Q Sepharose column chromatography, and Superdex 75-pg gel filtration | Candolleomyces aberdarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
82.9 | - |
isoform UPO-II, with veratryl alcohol as substrate, at pH 6.0 and 25°C | Candolleomyces aberdarensis |
205.9 | - |
isoform UPO-II, with veratryl alcohol as substrate, at pH 7.0 and 25°C | Candolleomyces aberdarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
5-nitro-1,3-benzodioxole + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
benzyl alcohol + H2O2 | - |
Candolleomyces aberdarensis | benzaldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Candolleomyces aberdarensis | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Candolleomyces aberdarensis |
? | x * 45000, SDS-PAGE | Candolleomyces aberdarensis |
? | x * 36500, calculated from amino acid sequence | Candolleomyces aberdarensis |
? | x * 37700, calculated from amino acid sequence | Candolleomyces aberdarensis |
? | x * 41247, MALDI-TOF mass spectrometry | Candolleomyces aberdarensis |
? | x * 42491, MALDI-TOF mass spectrometry | Candolleomyces aberdarensis |
Synonyms | Comment | Organism |
---|---|---|
UPO-I | isoform | Candolleomyces aberdarensis |
UPO-II | isoform | Candolleomyces aberdarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
mutant S61A/L79I/A252L of isoform UPO-I retains 50% of its activity after 10 min incubation at 56°C | Candolleomyces aberdarensis |
61 | - |
isoform UPO-II retains 50% of its activity after 10 min incubation at 61°C | Candolleomyces aberdarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
22 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
27 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
29 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
33 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
87 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
97 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
106 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
114 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
117 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
214 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
254 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
isoform UPO-II | Candolleomyces aberdarensis |
6 | - |
mutant S61A/L79I/A252L of isoform UPO-I | Candolleomyces aberdarensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | mutant S61A/L79I/A252L of isoform UPO-I retains over 40% of its relative activity in the pH range from 3.0 to 9.0 | Candolleomyces aberdarensis |
4 | 7 | isoform UPO-II retains over 40% of its relative activity in the pH range from 4.0 to 7.0 | Candolleomyces aberdarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Candolleomyces aberdarensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candolleomyces aberdarensis | calculated from amino acid sequence | - |
5.1 |
Candolleomyces aberdarensis | calculated from amino acid sequence | - |
5.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
26 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
32 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
62 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
67 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
76 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
112 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
219 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
277 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
308 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
582 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
679 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis |